Tobacco rattle virus–induced gene silencing in <i>Hevea brasiliensis</i>

ABSTRACT Virus-induced gene silencing (VIGS) is a powerful gene-silencing tool that has been intensively applied in plants. To data, the application of VIGS rubber tree not yet reported. In this study, we described efficient by VIGS. The encoding Hevea brasiliensis phytoene desaturase (HbPDS) was identified genome. Small interfering RNAs from HbPDS and fragment were predicted length 399 bp selected to be tested. We showed tobacco rattle virus (TRV)-VIGS could induce effective tree. This study first report present TRV-VIGS method used perform reverse genetic approaches identify unknown functions might further produce silenced plants, advance functional